IL-33 Protein, Human
IL-33 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | IL-33 |
| Accession | O95760 |
| Amino Acid Sequence | Ser112-Thr270 |
| Expression System | E.coli |
| Molecular Weight | 18-19kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE&RP-HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50mM Tris, 50mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
IL-33 is a nuclear cytokine from the IL-1 family constitutively expressed in epithelial barrier tissues and lymphoid organs, which plays important roles in type-2 innate immunity and human asthma. Recent studies indicate that IL-33 induces production of large amounts of IL-5 and IL-13 by group 2 innate lymphoid cells (ILC2s), for initiation of allergic inflammation shortly after exposure to allergens or infection with parasites or viruses. IL-33 appears to function as an alarmin (alarm signal) rapidly released from producing cells upon cellular damage or cellular stress.
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Bioactivity
SDS-PAGE
RP-HPLC
ELISA
Immobilized IL-33, Human (Cat. No. UA040036) at 2.0μg/mL (100μL/well) can bind IL1RL1/ST2 Fc Chimera, Human (Cat. No. UA010464) with EC50 of 4.42-4.97 ng/mL.
SPR
Protein A Chip captured IL1RL1/ST2 Fc Chimera, Human (Cat. No. UA010464), can bind IL-33, Human (Cat. No. UA040036) with an affinity constant of 1.25nM as determined in SPR assay .
