IL-2R alpha/CD25 Fc Chimera Protein, Human
IL-2R alpha/CD25 Fc Chimera Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL-2R alpha, CD25, IL2RA, p55, IL2-RA, IL-2-RA |
| Accession | P01589 |
| Amino Acid Sequence | Glu22-Cys213, with C-terminal Human IgG1 Fc |
| Expression System | HEK293 |
| Molecular Weight | 55-72kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1、Nelson B H. et al. (1998) Biology of the interleukin-2 receptor. Adv Immunol. 70: 1-81. 2、Schorle H. et al. (1991) Development and function of T cells in mice rendered interleukin-2 deficient by gene targeting. Nature. 352: 621-624. 3、Willerford D M. et al. (1995) Interleukin-2 receptor alpha chain regulates the size and content of the peripheral lymphoid compartment. Immunity. 3: 521-530. |
Background
Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. The interaction of interleukin-2 (IL-2) with its receptor (IL-2R) critically regulates the T-cell immune response, and the α chain CD25/IL-2Rα is required for the formation of the high-affinity receptor. Among them, IL-2 plays a major role in the regulation of the magnitude and duration of T-cell activation in the immune responses. IL-2 promotes T-cell proliferation by interacting with its high-affinity receptor composed of three transmembrane polypeptides, the α, β, and γc chains. The α chain (CD25) is induced during T-cell activation, and its association with β and γc chains constitutes the high-affinity receptor. Most of the biologic effects of IL-2 are mediated through the high-affinity complex as evidenced by the almost identical phenotype of mice with targeted inactivation of the genes for IL-2 and IL-2Rα.
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SDS-PAGE
ELISA
Immobilized IL-2 His Tag, Human (Cat. No. UA040057) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 1.23-1.99 ng/ml.
Immobilized IL-2 Protein, Human (Cat. No. UA040108) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 0.78-1.01ng/mL.
SPR
Protein A Chip captured IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260), can bind IL-2 His Tag, Human (Cat. No. UA040057) with an affinity constant of 15.55nM as determined in SPR assay.
