IL-2 Protein, Human
IL-2 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | IL-2 |
| Synonyms | Interleukin-2 |
| Accession | P60568 |
| Amino Acid Sequence | Ala21-Thr153 |
| Expression System | E.coli |
| Molecular Weight | 15.5 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Pol, J. G. , Caudana, P. , Paillet, J. , Piaggio, E. , & Kroemer, G. . (2020). Effects of interleukin-2 in immunostimulation and immunosuppression. The Journal of Experimental Medicine(1), 217. 2.Kolios, A. G., Tsokos, G. C., & Klatzmann, D. (2021). Interleukin-2 and regulatory T cells in rheumatic diseases. Nature Reviews Rheumatology, 17(12), 749-766. 3.Zhang, Y., & Su, J. (2023). Interleukin-2 family cytokines: An overview of genes, expression, signaling and functional roles in teleost. Developmental & Comparative Immunology, 104645. |
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self-antigens", which could result in "autoimmunity". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
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Bioactivity
SDS-PAGE
RP-HPLC
ELISA
Immobilized IL-2 Protein, Human (Cat. No. UA040108) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 His Tag Protein, Human (Cat. No. UA010367) with EC50 of 46.89-66.25ng/mL.
Immobilized IL-2 Protein, Human (Cat. No. UA040108) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 0.78-1.01ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured IL-2R alpha/CD25 His Tag, Mouse (Cat. No. UA010324), can bind IL-2, Human (Cat. No. UA040108) with an affinity constant of 20.57 nM as determined in SPR assay.
Anti-His antibody Immobilized on CM5 Chip captured IL-2R alpha/CD25 His Tag, Human (Cat. No. UA010367), can bind IL-2, Human (Cat. No. UA040108) with an affinity constant of 13.94 nM as determined in SPR assay.
Anti-His antibody Immobilized on CM5 Chip captured IL-2R beta/CD122 His Tag, Human (Cat. No. UA010372), can bind IL-2, Human (Cat. No. UA040108) with an affinity constant of 0.32 μM as determined in SPR assay.
