Measured by its ability to enhance IFN-γ secretion in KG-1 human myelomonocyte cells. The EC50 for this effect is less than 10ng/ml.
IL-18 Protein, Human
IL-18 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | IL-18 |
| Synonyms | IGIF, IL-18, IL1F4 |
| Accession | Q14116 |
| Amino Acid Sequence | Tyr37-Asp193 |
| Expression System | E.coli |
| Molecular Weight | 18 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1. Acta Biochim Pol 2016;63(1):59-63. Epub 2016 Feb 17. |
Background
Interleukin 18 (IL-18) is a pleiotropic cytokine involved in the regulation of innate and acquired immune response. In the milieu of IL-12 or IL-15, IL-18 is a potent inducer of IFN-gamma in natural killer (NK) cells and CD4 T helper (Th) 1 lymphocytes. However, IL-18 also modulates Th2 and Th17 cell responses, as well as the activity of CD8 cytotoxic cells and neutrophils, in a host microenvironment-dependent manner. IL-18 is a cytokine that stimulates various cell types and has pleiotropic functions. Recently, IL-18 has also been shown to execute specific effects in pancreatic diseases, including acute and chronic pancreatitis, as well as pancreatic cancer.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Measured by its binding ability in a functional ELISA. When IL-18Rβ/IL-1R7 His Tag, Human (Cat. No. UA010888) is immobilized at 2.0µg/mL (100 µL/well), in the present of IL-18, Human (Cat. No. UA040111), IL-18Rα/IL-1R5 Fc Chimera, Human (Cat. No. UA010874) binds with an ED50 of 8.62-11.50 ng/mL.
When IL-18Rβ/IL-1R7 Fc Chimera Protein, Human (Cat. No. UA010873) is immobilized at 2.0µg/mL (100 µL/well), in the present of IL-18, Human (Cat. No. UA040111), IL-18Rα/IL-1R5 His Tag Protein, Human (Cat. No. UA010914) binds with an ED50 of 40.10-49.63ng/mL.
Immobilized IL-18, Human (Cat. No. UA040111) at 2.0μg/mL (100μL/well) can bind IL-18BP Fc Chimera, Human (Cat. No. UA010587) with EC50 of 2.20-3.25 ng/mL
Immobilized IL-18, Human (Cat. No. UA040111) at 2.0μg/mL (100μL/well) can bind IL-18BP His Tag, Human (Cat. No. UA010574) with EC50 of 0.68-0.91 ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured IL-18BP His Tag, Human (Cat. No. UA010574), can bind IL-18, Human Cat. No. UA040111) with an affinity constant of 3.15nM as determined in SPR assay.
CM5 Chip captured IL-18Rα/IL-1R5 His Tag, Human (Cat. No. UA010914), can bind IL-18, Human (Cat. No. UA040111) with an affinity constant of 1.20nM as determined in SPR assay.
