The EC50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 was found to be <0.2ng/ml.
IL-15 Protein, Human
IL-15 Protein, Human
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$240.00 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | IL-15 |
| Accession | P40933 |
| Amino Acid Sequence | Asn49-Ser162 |
| Expression System | E.coli |
| Molecular Weight | 12 kDa(Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Interleukin-15 (IL-15), a cytokine discovered in 1994, supports the homeostasis of cytotoxic immune cells, exhibits a broad biological activity. IL-15 stimulate the proliferation of activated T cells as well as to facilitate the induction of cytotoxic T-lymphocytes (like CD8+), and the generation, proliferation, and activation of NK cells. Therefore, IL-15 is applied to the rapidly expanding cancer immunotherapy field combining with other agents, i.e., checkpoint inhibitors, monoclonal antibodies, chemotherapy, radiation, and chimeric antigen receptor T (CAR-T) cells, in order to target multiple mechanisms and enhance the immune response against tumors, which provides advantages to control and treat cancers.
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Bioactivity
SDS-PAGE
1μg (N: non-reducing condition, R: reducing condition).
ELISA
Immobilized IL-15, Human (Cat. No. UA040010) at 2μg/mL (100μL/well) can bind IL-15R alpha/CD215 Fc Chimera, Mouse (Cat. No. UA010314) with EC50 of 18.40-68.11 ng/mL.
Immobilized IL-15 Protein, Human (Cat. No. UA040010) at 2.0μg/mL (100μL/well) can bind IL-15R alpha/CD215 Fc Chimera Protein, Human (Cat. No. UA010309) with EC50 of 6.27-8.97ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured IL-2R beta/CD122 His Tag, Human (Cat. No. UA010372), can bind IL-15, Human (Cat. No. UA040010) with an affinity constant of 19.04 nM as determined in SPR assay.
Protein A Chip captured IL-15R alpha/CD215 Fc Chimera, Human (Cat. No. UA010309), can bind IL-15, Human (Cat. No. UA040010) with an affinity constant of 4.35 nM as determined in SPR assay.
