Mouse IL-4 ELISA Kit

Catalog Number: abs520003 Application: ELISA Reactivity: Mouse Conjugation:

Price:

$450 USD

Size: 96T

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Product Details

Product Specification

Theory

Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-4 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the well of microplate. After incubation, IL-4 present in the sample combined with solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm).

Composition

Component	Size	Once opened, diluted or heavy soluble reagent expiration date
Mouse IL-4 Microplate	1	Unused slats can be stored at 2-8 ° C for up to 30 days after being sealed back in aluminum foil bags with desiccants
Mouse IL-4 standard substance	2	Dissolves, calculate the dosage repackaging, can be in - 20 ° C storage for 14 days
Mouse IL-4 detect antibody	1	Enrichment dissolved after the volume, can be in 2-8 ° C storage for 14 days
40×SA-HRP	1	40× concentration can be stored at 2-8°C; 1 x working concentration is not recommended to save
10 x condense dilute with buffer	1	After opening, can be in 2-8 ° C stored for 30 days
Color-substrate solution	1
Stop buffer	1
20 x inspissation washing buffer	1
Sealing film	3	Room temperature storage, to avoid contamination, do not repeat use

Background

Interleukin-4 (IL-4), also known as B cell stimulating factor 1, is a monomeric cytokine of about 18-20 kda that has pleiotropic effects in immune responses. The precursor of mouse IL-4 is 140 amino acids, including a 20-amino acid signal peptide and a 120-amino acid mature chain. IL-4 contains three N-linked glycosylation potential sites, three intra-chain disulphide bonds, and its structure is a bundled tetra-α-spirochetes. Similar to human IL-4, mouse IL-4 has a small selective splicer. "Unlike human IL-4, the expression of this spliceosome is thought to be low and of little significance." The amino acid sequence of mature mouse IL-4 shared 43% and 63% identity with human and rat IL-4, respectively. Studies have shown that the activities of human, mouse and rat IL-4 are species specific. IL - 4 by toward Th2 CD4 + T cells, basophils and mast cells and NKT cells, gamma delta T cells with eosinophilic cells expressed.
IL - 4 has played a key role in type 2 immune reaction, can promote the Th2 differentiation and B cell secretory immunoglobulin G1 and immunoglobulin E subtypes. IL-4 causes Th2 differentiation by binding to type I or type II receptor complexes containing IL-4 receptor alpha subunits coupled to the γ chain or IL13Rα1, respectively. IL - 4 by cell surface receptors of different dimers, activate JAK family of protein complex amino acid kinase, cause its cytoplasm C phosphorylation. This triggers the recruitment and phosphorylation of STAT6. The phosphorylation and conformational changes of STAT6 lead to dimerization, nuclear translocation, binding and transcriptional activation of STAT6 target genes, including IL-4, IL-5, IL-13, and Th2-specific cytokines GATA3 and c-Maf. On the function, IL - 4 B cell proliferation, survival, and promote mice immune globulin class into IgG1 and IgE, promote the mast cells, eosinophils, and basophils start and chemokines, promote Th2 phenotype naive CD4 + cells, and promote the epithelial cell proliferation and activation. IL-4 is also an important cytokine in tumor immunology. In early experiments in mice, the researchers found that IL-4 had potent anti-tumor abilities. Mice reject tumors expressing IL-4 and develop long-term antitumor immunity. This may be due to the antiangiogenic effect of IL-4 or its ability to activate, select for CD8+T cells. "Paradoxically, emerging evidence suggests that IL-4 is a tumor-promoting molecule, and therefore, IL-4 is a reverse-acting cytokine." "A possible explanation is that IL-4 induces upregulation of antiapoptotic molecules in tumor cells and downregulation of cytolytic molecules in CD8+T cells." In addition, tumor-produced IL-4 is also thought to act on local tumor-associated macrophages (TAMs) to induce the secretion of cathepsins that promote cell migration.

General Notes

1. Please use the kit within the validity period.
2. Components of different kits and different batch kits should not be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. "If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used."
4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.

Storage Temp.

Unopened kit, 2-8° C Storage

Test Range

15.6pg/mL-1000pg/mL

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Mouse IL-4 ELISA Kit