WB result of XPO1/CRM1 Recombinant Rabbit mAb
Primary antibody: XPO1/CRM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 110 kDa
XPO1/CRM1 Recombinant Rabbit mAb (S-3309-7)
XPO1/CRM1 Recombinant Rabbit mAb (S-3309-7)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | XPO1/CRM1 |
| Synonyms | Exportin-1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O14980 |
| Clone Number | S-3309-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, MCF7, Jurkat, K562, NIH/3T3, RAW264.7, PC-12, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
XPO1 (Exportin-1, also known as CRM1) is an evolutionarily highly conserved nuclear transport receptor belonging to the karyopherin-β family, primarily responsible for recognizing and binding cargo proteins containing leucine-rich nuclear export signals (NES)—including critical tumor suppressors such as p53, p21, and FOXO, as well as various oncogenic mRNAs—and actively transporting them from the nucleus to the cytoplasm in a RanGTP-dependent manner, thereby precisely regulating cell growth, division, stress responses, and apoptosis. As the "gatekeeper" of intracellular transport, XPO1 dysfunction leads to the erroneous retention and inactivation of tumor suppressors in the cytoplasm, promoting unlimited cancer cell proliferation and survival; consequently, it has emerged as a pivotal therapeutic target in oncology, where Selective Inhibitors of Nuclear Export (SINEs, e.g., Selinexor) exert their effects by covalently binding to XPO1's cargo-binding groove to block its function, forcing the re-accumulation and reactivation of tumor suppressors within the nucleus, ultimately inducing cancer cell apoptosis.
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Western Blot
WB result of XPO1/CRM1 Recombinant Rabbit mAb
Primary antibody: XPO1/CRM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 110 kDa
WB result of XPO1/CRM1 Recombinant Rabbit mAb
Primary antibody: XPO1/CRM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 110 kDa
WB result of XPO1/CRM1 Recombinant Rabbit mAb
Primary antibody: XPO1/CRM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix squamous cell carcinoma. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-XPO1/CRM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-XPO1/CRM1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
