UA-Glo® Bright Luciferase Assay System
UA-Glo® Bright Luciferase Assay System
Product Details
Product Details
Product Specification
| Synonyms | High-luminosity Luciferase Detection Kit |
| Stability & Storage |
Reagents stored at 20°C that cannot be used up completely once opened are recommended to be aliquoted and then frozen at 20°C. It is recommended that freeze-thaw cycles do not exceed 3 times, and each time the reagent is left at room temperature, the duration should not exceed 1 hour. |
Background
The UA-Glo High Luminosity Luciferase Assay Kit is used for the quantitative detection of the content of stably expressed luciferase in cells. This reagent features high signal-to-noise ratio, good repeatability, and excellent stability. There is no need to remove the culture medium; the detection reagent can be directly added to the culture plate for detection, thereby further reducing operational errors. Moreover, its stable signal makes this product particularly suitable for high-throughput sample detection. Compared with the Steady Glo stable luciferase assay kit, the Bright Glo high luminosity luciferase assay kit has a detection signal several times higher, which can meet the needs of high-sensitivity detection.
Components
ATP, luciferin, and buffer solution are mixed and then filled into 10 ml or 100 ml brown bottles, with the specifications as follows:
|
Product Specifications |
Can detect the number of wells in a 96-well plate |
Can detect the number of wells in a 384-well plate |
|
10 ml |
100 |
500 |
|
100 ml |
1,000 |
5,000 |
|
10 x 100 ml |
10,000 |
50,000 |
Protocol
1. Before the experiment, equilibrate the UA-Glo high-luminosity luciferase detection reagent to room temperature and mix gently by shaking.
2. Equilibrate the cell plate to be tested (white opaque-bottom or black opaque-bottom plate) at room temperature for 10 minutes.
3. Add the detection reagent in a volume equal to that of the cell culture medium to each culture reaction well.
4. Shake on a plate shaker for 2 minutes, then incubate the sample plate at room temperature in the dark for 5-8 minutes.
5. Read and record the fluorescence signal on a luminescence microplate reader. It is recommended to complete the plate reading within 10-30 minutes to obtain the best results.
