WB result of Pescadillo Rat mAb Primary antibody: Pescadillo Rat mAb at 1/4000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Lane 3: HEK-293 whole cell lysate 20 µg Lane 4: HCT 116 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 68 kDa Observed MW: 70 kDa
PES1 Recombinant Rat mAb (SDT-R197)
PES1 Recombinant Rat mAb (SDT-R197)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | PES1 |
| Synonyms | Pescadillo homolog |
| Immunogen | N/A |
| Location | Nucleus, Nucleolus |
| Accession | O00541 |
| Clone Number | SDT-R197 |
| Antibody Type | Rat mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC, ICFCM, IP |
| Reactivity | Hu |
| Concentration | 0.4 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:4000 | null |
| IHC | 1:400 | null |
| ICC | 1:100 | null |
| ICFCM | 1:500 | null |
| IP | 1:40 | null |
Background
Pescadillo homolog is a protein that in humans is encoded by the PES1 gene [PMID: 8985183]. It is a nuclear protein that is involved in embryonic development, ribosome synthesis, DNA replication, and cell cycle progression [PMID: 31814761].
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Picture
Western Blot
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PES1 at 1/500 dilution (0.1 μg) / (red) compared with a Rat monoclonal IgG isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
PES1 Rat mAb at 1/40 dilution (1 µg) immunoprecipitating PES1 in 0.4 mg HEK-293 whole cell lysate.
Western blot was performed on the immunoprecipitate using PES1 Rat mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate 20 µg (Input)
Lane 2: PES1 Rat mAb IP in HEK-293 whole cell lysate
Lane 3: Rat monoclonal IgG1 IP in HEK-293 whole cell lysate
Predicted MW: 68 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-PES1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
