WB result of Olfactory Marker/OMP Recombinant Rabbit mAb
Primary antibody: Olfactory Marker/OMP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: Neuro-2a whole cell lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Lane 5: mouse kidney lysate 20 µg
Lane 6: mouse heart lysate 20 µg
Lane 7: mouse brain lysate 20 µg
Lane 8: mouse olfactory bulb lysate 20 µg
Negative control: NIH/3T3 whole cell lysate; RAW264.7 whole cell lysate; Neuro-2a whole cell lysate; mouse spleen lysate; mouse kidney lysate; mouse heart lysate; mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
Olfactory Marker/OMP Recombinant Rabbit mAb (S-3513-32)
Olfactory Marker/OMP Recombinant Rabbit mAb (S-3513-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Olfactory Marker/OMP |
| Synonyms | Olfactory marker protein; Olfactory neuronal-specific protein; OMP |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm |
| Accession | P47874 |
| Clone Number | S-3513-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms, Rt |
| Positive Sample | mouse olfactory bulb, rat olfactory bulb |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Ms, Rt |
| IF | 1:250 | Ms, Rt |
Background
Olfactory Marker Protein (OMP) is a small, ~19 kDa cytosolic protein uniquely and abundantly expressed in mature olfactory receptor neurons (ORNs) across the vertebrate olfactory epithelium, from the cell soma to the distal axon terminals in the olfactory bulb, where it was first discovered by Frank Margolis in the 1970s as a specific molecular signature for terminally differentiated olfactory neurons. Although its precise physiological function remains incompletely understood, OMP is believed to modulate olfactory signal transduction by interacting with odorant receptor signaling cascades, regulating cAMP kinetics, and potentially facilitating odorant adaptation, while also playing roles in axon guidance, synaptic targeting, and the refinement of olfactory maps during development and regeneration. As the definitive immunohistochemical marker for mature ORNs, OMP distinguishes fully differentiated neurons from immature (expressing GAP43) and sustentacular cells, making it indispensable for studying neuronal turnover, lesion-induced regeneration, and the functional anatomy of the olfactory system in both normal and pathological states, including neurodegenerative diseases and post-viral olfactory dysfunction.
Picture
Picture
Western Blot
WB result of Olfactory Marker/OMP Recombinant Rabbit mAb
Primary antibody: Olfactory Marker/OMP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Lane 4: rat olfactory bulb lysate 20 µg
Negative control: PC-12 whole cell lysate; C6 whole cell lysate; rat kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse olfactory bulb. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse spleen. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat olfactory bulb. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse olfactory bulb. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat olfactory bulb. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
