WB result of NNMT Recombinant Rabbit mAb
Primary antibody: NNMT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 25 kDa
NNMT Recombinant Rabbit mAb (S-3470-58)
NNMT Recombinant Rabbit mAb (S-3470-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NNMT |
| Synonyms | Nicotinamide N-methyltransferase |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm |
| Accession | P40261 |
| Clone Number | S-3470-58 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | A549, HeLa, HUVEC, |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
Background
Nicotinamide N-methyltransferase (NNMT) is a cytosolic metabolic enzyme encoded by a gene located on chromosome 11q23.2. Its core biochemical function is the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), generating 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH). This reaction positions NNMT as a pivotal nexus linking methylation metabolism, NAD⁺ synthesis, and epigenetic regulation. NNMT's sustained consumption of the SAM substrate not only limits methyl donor availability for DNA methyltransferases and histone methyltransferases—leading to loss of repressive histone marks such as H3K27me3 and global DNA hypomethylation—but also depletes the NAD⁺ precursor nicotinamide, thereby impairing mitochondrial respiration and the activity of deacetylases such as SIRT1. This accelerates mitochondrial dysfunction and neuronal apoptosis in neurodegenerative diseases, while within the tumor microenvironment, it drives the activation and differentiation of cancer-associated fibroblasts (CAFs) and recruits myeloid-derived suppressor cells (MDSCs) via the complement cascade to establish an immunosuppressive niche. Research has confirmed that NNMT is specifically upregulated by the glucocorticoid receptor signaling axis in glioblastoma, where its product 1-MNA is enriched approximately 7-fold in tumor tissue compared to adjacent normal tissue—a disparity now exploited for [¹¹C] nicotinamide PET imaging strategies. In hypertrophic scars, NNMT-mediated NAD⁺ and SAM depletion drives macrophage-to-myofibroblast transdifferentiation (MMT) through accumulation of H3K27ac and loss of H3K27me3; NNMT knockdown disrupts the interaction between the transcription factor Prrx1 and super-enhancers, significantly reducing scar volume.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
