| Host | Rabbit |
| Antigen | Keratin 14 |
| Synonyms | Cytokeratin-14,CK-14, KRT14 |
| Immunogen | Synthetic Peptide |
| Accession | P02533 |
| Clone Number | SDT-023-1 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms |
| Predicted Reactivity | Rt |
| Purification | Protein A |
| Research Area | Signal Transduction |
| Concentration | 0.5mg/ml |
| Molecular Weight | 51kDa |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IHC-P | 1:1000 | |
| ICC | 1:500 | |
| WB | 1:1000 | |
| IP | 1:25 |
Keratin 14 is a member of the type I keratin family of intermediate filament proteins. Keratin 14 was the first type I keratin sequence determined. Keratin 14 is also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14). In humans it is encoded by the KRT14 gene. Keratin 14 is expressed in mitotically active basal layer cells, along with its partner keratin 5 (K5), and their expression is down-regulated as cells differentiate. Keratin 14 has been studied as a prognostic marker in breast cancer. Keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and has been reported useful in the identification of squamous cell carcinomas.
WB result of Keratin 14 Rabbit mAb
Primary antibody : Keratin 14 Rabbit mAb at 1/1000 dilution
Lane 1 : A431 whole cell lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
Exposure time: 0.9 second
WB result of Keratin 14 Rabbit mAb
Primary antibody : Keratin 14 Rabbit mAb at 1/1000 dilution
Lane 1 : mouse skin lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 53 kDa
Exposure time: 0.9 seconds
Keratin 14 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Keratin 14 in 0.4mg A431 whole cell lysate. Western blot was performed on the immunoprecipitate using Keratin 14 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A431 whole cell lysate 10µg (input)
Lane 2: Keratin 14 Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 51 kDa
Observed MW: 51 kDa
Exposure time: 3s
IHC shows membrane staining in paraffin-embedded human skin squamous epithelium.
Anti-Keratin 14 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human tonsil squamous epithelium.
Anti-Keratin 14 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human prostate.
Anti-Keratin 14 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human cervix cancer. Anti-Keratin 14 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human lung squamous cell cancer.
Anti-Keratin 14 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded mouse skin squamous epithelium.
Anti-Keratin 14 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows membrane staining in A431 cells.
Anti-Keratin 14 antibody was used at 1/500 dilution and incubated overnight at 4°C.
Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.
The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100.
Nuclei were counterstained with DAPI.
