WB result of IRF3 Rabbit mAb
Primary antibody: IRF3 Rabbit mAb at 1/2000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 55 kDa
IRF3 Recombinant Rabbit mAb (S-449-24)
IRF3 Recombinant Rabbit mAb (S-449-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Interferon regulatory factor 3, IRF-3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q14653 |
| Clone Number | S-449-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000 | |
| IP | 1:50 | |
| IHC-P | 1:250 |
Background
IRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 plays an important role in the innate immune system's response to viral infection. It is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. The complex translocates into the nucleus for the transcriptional activation of interferons alpha and beta, and further interferon-induced genes.
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Picture
Western Blot
IP
IRF-3 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IRF-3 in 0.4 mg THP-1 whole cell lysate.
Western blot was performed on the immunoprecipitate using IRF-3 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: THP-1 whole cell lysate 10 µg (Input)
Lane 2: IRF-3 Rabbit mAb IP in THP-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in THP-1 whole cell lysate
Predicted MW: 47 kDa
Observed MW: 50 kDa
（This blot was developed with high sensitivity substrate）
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-IRF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IRF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-IRF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid carcinoma. Anti-IRF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
