| 1. Basic Product Information |
Full Product Name: hPSC-Induced Differentiation Kit for Cardiac Organoids
Cat. No.: abs90364, Standard Pack: abs90364-1kit
Brand: Absin (Absin Bioscience Inc., Shanghai)
Applicable Cells: Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs)
Usage Restriction: For research use only. Not for clinical diagnosis or human therapeutic administration.
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| 2. Core Product Description |
This all-in-one 4-stage kit enables 3D cardiac organoid differentiation, fully recapitulating the in vivo developmental microenvironment of the human heart. No separate preparation of growth factors or small-molecule inhibitors is required. The standardized 20-day differentiation workflow generates self-cavitated, spontaneously contracting human cardiac organoids.
Differentiated products contain multiple native cardiac cell populations: atrial/ventricular cardiomyocytes, epicardial cells, endothelial cells and cardiac fibroblasts. The cell composition and spatial architecture closely mimic early human cardiac tissue. The organoids support long-term maturation culture, electrophysiological assays, cardiotoxicity testing of pharmaceuticals, in vitro modeling of congenital heart diseases, and mechanistic research on cardiac development.
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| 3. Kit Components, Specifications, Storage & Shelf Life |
The kit includes stage-specific basal media, supplements, and a large-volume maturation maintenance medium for the four differentiation phases.
| Differentiation Stage |
Culture Duration |
Component Name |
Volume |
Storage Condition |
Shelf Life |
| Stage I (Mesoderm Induction) |
Day 0–Day 2 |
Basal Medium 1 + Supplement A |
9.98 mL + 20 μL |
-20°C, protected from light |
12 months for basal medium; 6 months for supplements |
| Stage II (Cardiac Mesoderm Specification) |
Day 3–Day 4 |
Basal Medium 2 + Supplement B |
9.94 mL + 60 μL |
-20°C, protected from light |
12 months for basal medium; 6 months for supplements |
| Stage III (Cardiomyocyte Lineage Differentiation) |
Day 5–Day 6 |
Basal Medium 3 + Supplement C |
9.98 mL + 20 μL |
-20°C, protected from light |
6 months for both medium and supplement |
| Stage IV (Cardiac Organoid Maturation & Maintenance) |
Day 7–Day 20 |
Cardiac Organoid Maturation Medium |
200 mL |
-20°C, protected from light |
12 months |
Pre-Use Preparation Guidelines
Add the corresponding supplement to the matching basal medium and mix thoroughly right before use. Prepared working medium can be stored at 2–8°C for up to 7 days.
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| 4. Standard Differentiation Timeline |
Day 0–Day 2: Stage I medium induces mesoderm formation; U-bottom ultra-low-adhesion 96-well plates support spontaneous 3D embryoid body generation.
Day 3–Day 4: Stage II medium directs cardiac mesoderm specification.
Day 5–Day 6: Stage III medium drives cardiomyocyte lineage differentiation.
Replace with Stage IV maturation medium starting on Day 7. Mature contracting cardiac organoids are obtained by Day 20. Extended culture up to 35+ days is available for functional assays.
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| 5. Key Product Advantages |
- Ready-to-use complete kit: Pre-formulated basal media plus concentrated aliquoted supplements eliminate separate procurement, dilution and formulation of multiple cytokines and small molecules, ensuring high experimental reproducibility.
- Physiologically relevant multi-cell cardiac structure: Produces not only cardiomyocytes but also epicardial, endothelial and fibroblast populations with native cardiac luminal cavities.
- Stable spontaneous contraction: Rhythmic autonomous beating is observable from Day 9–Day 13, enabling direct quantitative analysis of beating rate and amplitude after drug stimulation.
- Standardized high-throughput production: One kit supports mass generation of cardiac organoids compatible with 96-well high-throughput drug screening.
- Low batch-to-batch variation: Industrially manufactured with strict quality control to stabilize differentiation efficiency and organoid maturation across batches.
- Long-term culture support: 200 mL large-volume maturation medium sustains functional culture for over 35 days.
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| 6. Primary Research Applications |
- In vitro research on early human cardiac developmental mechanisms
- Construction of in vitro disease models for congenital heart defects
- High-throughput cardiotoxicity screening of small-molecule drugs and chemical toxicants
- Pharmacological, efficacy and electrophysiological safety evaluation of novel cardiovascular therapeutics
- Basic research on myocardial injury repair and cardiac regeneration
- Functional validation of pathogenic genes via CRISPR gene editing
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| 7. Recommended Matching Consumables |
- U-bottom ultra-low-adhesion 96-well cell culture plates (for embryoid body formation)
- Absin high-concentration phenol-red-free Matrigel (Cat. abs9493) for hPSC coating
- Cell dissociation solution and ROCK inhibitor to improve stem cell viability
- Flow cytometry & immunofluorescence antibodies for cardiac cell identification: cTNT, CD31, WT1, etc.
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| 8. Storage & Operation Precautions |
- Store the unopened complete kit at -20°C away from light; avoid repeated freeze-thaw cycles.
- Aliquot supplements into small volumes; return unused portions to freezer promptly after each use.
- Do not freeze prepared working medium; short-term storage is limited to 2–8°C refrigeration.
- Operators must possess established experience in hPSC cell culture.
- Strict aseptic handling required; all consumables and reagents are endotoxin-free and mycoplasma-negative.
- Differentiated organoids are solely for in vitro research; implantation into animals or human subjects is prohibited.
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| Usage |
For research use only |
| Storage Temp. |
Unopened kit: -20°C, protected from light; Prepared working medium: 2–8°C (max 7 days) |
