HDAC1 Recombinant Rabbit mAb (SDT-310-72)

Catalog Number: S0B0090 Application: WB,IHC-P,ICC,FCM,IP Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100 μl

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Product Details

Product Specification

Host	Rabbit
Antigen	HDAC1
Synonyms	Histone deacetylase 1, HD1, Protein deacetylase HDAC1
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	Q13547
Clone Number	SDT-310-72
Antibody Type	Recombinant mAb
Application	WB, IHC-P, ICC, ICFCM, IP
Reactivity	Hu, Ms, Rt
Predicted Reactivity	Bv
Purification	Protein A
Concentration	0.05 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied.

Dilution

application	dilution	species
WB	1:1000-1:5000
IHC-P	1:200
ICC	1:50
ICFCM	1:50
IP	1:25

Background

HDAC family has four subclasses including I, II, III and IV. Histone deacetylase 1 (HDAC1) is a unique member of the classes I HDACs that has been shown to be involved in gene transcription, transcriptional regulation, cell cycle progression and developmental events by controlling both enzyme activity and epigenetics of key proteins [PMID: 16289629]. HDAC1 is the most abundant member of the class I HDACs in pulmonary endothelial cells [PMID: 34264338], regulating the enzymatic activity and epigenetics of key proteins to adapt to external stimuli. It can efficiently decrotonylate this relatively less abundant histone modification [PMID: 29317660].

Picture

Western Blot

WB result of HDAC1 Rabbit mAb Primary antibody: HDAC1 Rabbit mAb at 1/5000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Lane 3: K-562 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55kDa Observed MW: 62kDa

WB result of HDAC1 Rabbit mAb Primary antibody: HDAC1 Rabbit mAb at 1/5000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55kDa Observed MW: 62kDa

WB result of HDAC1 Rabbit mAb Primary antibody: HDAC1 Rabbit mAb at 1/5000 dilution Lane 1: C6 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55kDa Observed MW: 62kDa

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HDAC1 antibody at 1/50 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

IP

HDAC1 Rabbit mAb at 1/25 dilution (0.2 µg) immunoprecipitating HDAC1 in 0.2 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using HDAC1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HeLa whole cell lysate 5 µg (Input)
Lane 2: HDAC1 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 55 kDa
Observed MW: 62 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-HDAC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-HDAC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-HDAC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-HDAC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-HDAC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti-HDAC1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).