Fixable Viability Dye 777

For reconstitution, pre-warm the kit to room temperature; add 100 µL of DMSO to one vial of Fixable Viability Dye 777 and mix until fully dissolved. After reconstitution, store aliquots at -20°C. Repeated freezing and thawing is not recommended.

For recommended usage, add 1 µl of Fixable Viability Dye 777 Stock Solution for each 5 ml of cell suspension/PBS (1:5000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.

Please refer to the protocol for details.

Human PBMC (human peripheral blood mononuclear cells) were stained with Fixable Viability Dye 777 and analyzed before fixation (Middle Panels) or after fixation (Right Panels). Cells alone, without Fixable Viability Dye 777 staining (Left Panels). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.