SD Rat splenocytes were stained with SDT FITC Mouse IgG2a, κ Isotype Control Antibody (Right panel) at 1/20 dilution (1 μg). Cells without incubation with primary antibody and secondary antibody (Left panel) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
Host | Mouse |
Immunogen | Recombinant Protein |
Clone Number | S-844-17 |
Antibody Type | Mouse mAb |
Isotype | IgG2a,k |
Application | FCM |
Purification | Protein A |
Concentration | 0.2 mg/ml |
Conjugation | FITC |
Physical Appearance | Liquid |
Storage Buffer | PBS, 0.3% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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