WB result of CDK7 Recombinant Rabbit mAb
Primary antibody: CDK7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SK-BR-3 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
CDK7 Recombinant Rabbit mAb (S-2739-124)
CDK7 Recombinant Rabbit mAb (S-2739-124)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CDK7 |
| Synonyms | Cyclin-dependent kinase 7; 39 kDa protein kinase (p39 Mo15); CDK-activating kinase 1; Cell division protein kinase 7; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunit; CAK; CAK1; CDKN7; MO15; STK1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P50613 |
| Clone Number | S-2739-124 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, HepG2, MCF7, SK-BR-3, HCT 116 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:4000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:2000 | Hu |
Background
CDK7 (Cyclin-Dependent Kinase 7) is a serine/threonine kinase that serves as a master regulator of the cell cycle and gene transcription, functioning as the catalytic subunit of the TFIIH (Transcription Factor II H) complex and the CAK (CDK-Activating Kinase) complex. As a component of TFIIH, CDK7, in association with its regulatory partner cyclin H and the MAT1 assembly factor, phosphorylates the C-terminal domain (CTD) of RNA polymerase II at serine 5 and serine 7 residues during transcription initiation, thereby facilitating promoter clearance and the transition to productive elongation. Simultaneously, as CAK, CDK7 activates other cell cycle CDKs (CDK1, CDK2, CDK4, and CDK6) through phosphorylation of a conserved threonine residue in their T-loops, thereby driving cell cycle progression from G1 through M phase. Beyond these canonical functions, CDK7 has been implicated in the regulation of transcriptional programs essential for cell growth, proliferation, and survival, particularly in cancer cells where its overexpression or hyperactivation is frequently observed. Consequently, CDK7 has emerged as a promising therapeutic target in oncology, with selective small-molecule inhibitors such as THZ1 and SY-1365 demonstrating potent anti-tumor activity in preclinical models by disrupting both cell cycle control and transcriptional addiction in malignancies including triple-negative breast cancer, small cell lung cancer, and acute myeloid leukemia.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDK7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CDK7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-CDK7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HCT116 cells. Anti-CDK7 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
