CD73 Recombinant Rabbit mAb (SDT-218-52)

Catalog Number: S0B2139 Application: WB,IHC-P Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 500μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	CD73
Synonyms	5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	P21589
Clone Number	SDT-218-52
Antibody Type	Rabbit mAb
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:500-1:1000
WB	1:1000

Background

CD73, also known as ecto-5′-nucleotidase (ecto-5′-NT, EC 3.1.3.5) is a glycosylphosphatidyl inositol (GPI)-anchored cell surface protein that is encoded by NT5E gene. CD73 is widely expressed on different tissues [PubMed: 9553767, PubMed: 18404475] and cell types including, but not limited to the subsets of T cells and B cells [PubMed: 2550543], endothelial cells [PubMed: 9015312, PubMed: 15358667] and epithelial cells [PubMed: 9169488].

Picture

Western Blot

WB result of CD73 Rabbit mAb
Primary antibody: CD73 Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 70 kDa
Exposure time: 120s

WB result of CD73 Rabbit mAb
Primary antibody: CD73 Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 68 kDa
Exposure time: 180s

WB result of CD73 Rabbit mAb
Primary antibody: CD73 Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 70 kDa
Exposure time: 180s

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-CD73 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD73 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-CD73 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.