Carbonic Anhydrase IX/CA9 His Tag Protein, Mouse
Carbonic Anhydrase IX/CA9 His Tag Protein, Mouse
Product Details
Product Details
Product Specification
| Species | Mouse |
| Antigen | Carbonic Anhydrase IX/CA9 |
| Synonyms | EC:4.2.1.1，Renal cell carcinoma-associated antigen G250，P54/58N，Carbonic Anhydrase 9，RCC-Associated Protein G250，Carbonate Dehydratase IX，Carbonic Dehydratase，CA-IX，CAIX，PMW1，MN，EC 4.2.1.1，G250，Membrane antigen MN，RCC-associated antigen G250 |
| Accession | Q8VHB5 |
| Amino Acid Sequence |
Gln32-Asp390, with C-terminal 8*His QPQGLSGMQGEPSLGDSSSGEDELGVDVLPSEEDAPEEADPPDGEDPPEVNSEDRMEESLGLEDLSTPEAPEHSQGSHGDEKGGGHSHWSYGGTLLWPQVSPACAGRFQSPVDIRLERTAFCRTLQPLELLGYELQPLPELSLSNNGHTVQLTLPPGLKMALGPGQEYRALQLHLHWGTSDHPGSEHTVNGHRFPAEIHVVHLSTAFSELHEALGRPGGLAVLAAFLQESPEENSAYEQLLSHLEEISEEGSKIEIPGLDVSALLPSDLSRYYRYEGSLTTPPCSQGVIWTVFNETVKLSAKQLHTLSVSLWGPRDSRLQLNFRATQPLNGRTIEASFPAAEDSSPEPVHVNSCFTAGDGGGSHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 43-55kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
·12 months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Supuran CT. Carbonic anhydrases: noveltherapeutic applications for inhibitors and activators. Nat Rev Drug Discov2008;7:168–81. 2.McDonald PC, Swayampakula M, Dedhar S..Coordinated regulation of metabolic transporters and migration/invasion bycarbonic anhydrase IX. Metabolites 2018;8:E20. |
Background
The CAIX works as a dimeric molecule composed of two monomeric proteins liked with a disulfide bond between cysteine residues of the two CAIX monomers, while the structure of CAXII is stabilized by 19 hydrogen-bonded interactions in the dimer interface. Carbonic anhydrases IX and CAXII (CAIX/CAXII) are transmembrane zinc metalloproteins that catalyze a very basic but crucial physiological reaction: the conversion of carbon dioxide into bicarbonate with a release of the proton.CAIX functions with bicarbonate transporters (including electroneutral bicarbonate transporter NBCn1 (SLC4A7)) to bring in HCO3- produced in a CAIX-mediated hydration reaction of CO2, and amino acid transporters (including the large neutral amino acid transporter 1 (LAT1) and CD98 heavy chain (CD98hc)) to transport in essential amino acids and glutamine (via interaction with glutamine transporter SLC1A5 and sodium-coupled neutral amino acid transporter 2 (SNAT2) facilitating the uptake of aminoacid by SLC1A5), which cancer cells employ as an alternative metabolic energy source and biosynthetic precursor. CAIX also creates unique interactions with collagen- and laminin-binding integrins at the leading edge of migrating cells.
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