| Host | Rabbit |
| Antigen | Calprotectin |
| Synonyms | Protein S100-A8, Calgranulin-A, MRP-8, S100 calcium-binding protein A8 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P05109 |
| Clone Number | SDT-049-44 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.1mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IHC-P | 1:3200 | null |
| WB | 1:200 | null |
| IP | 1:25 | null |
WB result of Calprotectin Rabbit mAb
Primary antibody: Calprotectin Rabbit mAb at 1/200 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 11 kDa
Calprotectin Rabbit mAb at 1/25 dilution (0.4 µg) immunoprecipitating Calprotectin in 0.4 mg THP-1 whole cell lysate.
Western blot was performed on the immunoprecipitate using Calprotectin Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: THP-1 whole cell lysate 5 µg (Input)
Lane 2: Calprotectin Rabbit mAb IP in THP-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in THP-1 whole cell lysate
Predicted MW: 11 kDa
Observed MW: 11 kDa
（This blot was developed with high sensitivity substrate）
IHC shows positive staining in paraffin-embedded human spleen. Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver.
Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer.
Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer.
Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen.
Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
