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c-Rel Recombinant Rabbit mAb (S-1150-52)

c-Rel Recombinant Rabbit mAb (S-1150-52)

Catalog Number: S0B0779 Application: WB,IHC-P,ICC,FCM,IP Reactivity: Mouse,Rat Conjugation: Unconjugated Brand: Starter
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Regular price $130.00 SGD
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Product Details

Product Specification


Host Rabbit
Antigen c-Rel
Synonyms Proto-oncogene c-Rel
Immunogen Synthetic Peptide
Location Cytoplasm, Membrane
Accession P15307
Clone Number S-1150-52
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P, ICC, ICFCM, IP
Reactivity Ms, Rt
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000
IHC-P 1:200
ICC 1:50
ICFCM 1:50
IP 1:50

Background

c-Rel is a member of the NF-κB family, predominantly expressed in lymphocytes and myeloid cells. As a transcription factor, it regulates the proinflammatory polarization of myeloid cells and modulates the antitumor immune response. Research has found that c-Rel serves as an important checkpoint for the immunosuppressive function of myeloid-derived suppressor cells (MDSCs). MDSCs hinder the normal function of immune cells in a tumor environment, promoting tumor immune evasion. c-Rel controls the polarization of myeloid cells in tumors, and specific inhibition of c-Rel significantly inhibits tumor growth. In c-Rel-deficient (Rel–/–) mice, melanoma and lymphoma tumor growth was significantly suppressed, with a 80% reduction in tumor size and body weight compared to controls. Inhibiting c-Rel through small molecule inhibitors or conditional knockout in MDSCs can promote the body's antitumor immune response. Combining c-Rel inhibitors with PD-1 functional blocking antibodies can further enhance the activation of the body's antitumor immune response.

Picture

Western Blot

WB result of c-Rel Recombinant Rabbit mAb
Primary antibody: c-Rel Recombinant mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Lane 4: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 70 kDa

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) labelling c-Rel antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

IP

c-Rel Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating c-Rel in 0.4 mg Neuro-2a whole cell lysate.
Western blot was performed on the immunoprecipitate using c-Rel Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Neuro-2a whole cell lysate 20 µg (Input)
Lane 2: c-Rel Rabbit mAb IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Neuro-2a whole cell lysate
Predicted MW: 65 kDa 
Observed MW: 70 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse spleen. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in Neuro-2a cells. Anti- c-Rel antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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