2μg (R: reducing condition, N: non-reducing condition).
Biotinylated CD7 His&Avi Tag Protein, Human
Biotinylated CD7 His&Avi Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD7, GP40, TP41, LEU-9, Tp40 |
| Accession | P09564-1 |
| Amino Acid Sequence | Ala26-Pro180, with C-10*His and Avi tag |
| Expression System | HEK293 |
| Molecular Weight | 28-40kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Sempowski G D. et al. (1999) Resistance of CD7-deficient mice to lipopolysaccharide-induced shock syndromes. J Exp Med. 189(6): 1011-1016. |
Background
CD7 is a 40-kD member of the Ig gene superfamily that is expressed on a major subset of human peripheral T lymphocytes and NK cells. CD7 is an early T cell activation antigen in that CD7 mRNA levels rise within 15 min after initiation of a transmembrane calcium ion flux. CD7 can complex with CD3 and CD45 molecules, and CD7 signaling involves both protein kinase C and protein tyrosine kinase. CD7 has been shown to be a functional signal-transducing molecule on resting NK cells. Antibody cross-linking of NK cell CD7 induced increases in free cytoplasmic calcium, secretion of IFN-γ, NK cell proliferation, adhesion to fibronectin, and NK cytotoxic activity. Although the above studies have demonstrated in vitro roles for CD7 in T and NK cell activation and/or adhesion, relevant functions of CD7 in vivo remain unknown.
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SDS-PAGE
ELISA
Immobilized Biotinylated CD7 His&Avi Tag Protein, Human (Cat. No. UA010725) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind CD7 Ligand/SECTM1 Fc Chimera, Human (Cat. No. UA010409) with EC50 of 1.03-1.61ng/ml.
Immobilized Biotinylated CD7 His&Avi Tag, Human (Cat. No. UA010725) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Grisnilimab (Cat. No. UA011033) with EC50 of 0.83-1.20 ng/ml.
