1μg (R: reducing condition, N: non-reducing condition).
Biotinylated B7-H4 Avi&His Tag Protein, Human
Biotinylated B7-H4 Avi&His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | B7-H4, VTCN1, B7S1, B7h.5 |
| Accession | Q7Z7D3-1 |
| Amino Acid Sequence | Phe29-Ala258, with C-terminal His and Avitag |
| Expression System | HEK293 |
| Molecular Weight | 43-60kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Yuping Sun, Yunshan Wang, Jianqiang Zhao, Ming Gu, Ricardo Giscombe, Ann Kari Lefvert a, Xiongbiao Wang: B7-H3 and B7-H4 expression in non-small-cell lung cancer, Lung Cancer Volume 53, Issue 2, August 2006, Pages 143-151. |
Background
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1/B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
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SDS-PAGE
ELISA
Immobilized Biotinylated B7-H4 Avi&His Tag Protein, Human (Cat. No. UA010852) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind B7-H4 Recombinant Rabbit mAb (SDT-314-447) (Cat. No. S0B2234) with EC50 of 2.12-5.11 ng/ml.
