Akt (pan) Recombinant Rabbit mAb (SDT-R169)

Catalog Number: S0B0114 Application: WB,IHC-P,IP Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

Price:

$100.00 USD

Size: 25ul 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	AKT1 + AKT2 + AKT3
Synonyms	N/A
Immunogen	N/A
Location	Cytoplasm, Nucleus, Cell membrane
Accession	P31749、 Q9Y243、P31751
Clone Number	SDT-R169
Antibody Type	Recombinant mAb
Application	WB, IHC-P, IP
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Tag	N/A
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	null
IHC-P	1:500-1:2000	null
IP	1:50	null

Background

Akt is a family of three serine-threonine kinases, Akt1, Akt2, and Akt3 [PMID: 28115590]. Akt is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. The Akt can be activated by a wide range of growth signals and the biochemical mechanisms leading to Akt activation are well defined [PMID: 31173856].

Picture

Western Blot

WB result of Akt (pan) Rabbit mAb
Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60kDa
Observed MW: 60kDa

WB result of Akt (pan) Rabbit mAb
Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60kDa
Observed MW: 60kDa

WB result of Akt (pan) Rabbit mAb
Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60kDa
Observed MW: 60kDa

IP

Akt (pan) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Akt (pan) in 0.4 mg MCF-7 whole cell lysate.
Western blot was performed on the immunoprecipitate using Akt (pan) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: MCF-7 whole cell lysate 20 µg (Input)
Lane 2: Akt (pan) Rabbit mAb IP in MCF-7 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in MCF-7 whole cell lysate
Predicted MW: 60 kDa
Observed MW: 60 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Akt (pan) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Akt (pan) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Akt (pan) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.