β-actin Recombinant Rabbit mAb (FITC conjugate) (SDT-R015)
β-actin Recombinant Rabbit mAb (FITC conjugate) (SDT-R015)
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | β-Actin |
| Synonyms | ACTB |
| Immunogen | N/A |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P60709 |
| Clone Number | SDT-R015 |
| Antibody Type | Recombinant mAb |
| Application | ICC, ICFCM |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:100 |
Background
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
