Competitive ELISA binding of 25-OH-VD3 recombinant mAb (S0B3603) with DNP-BSA conjugate.
Free 25-OH VD3 blocks 25-OH-VD3 recombinant mAb (S0B3603) binding with coating antigen (VD3-BSA conjugate)
Coating antigen: VD3-BSA conjugate, 0.5 µg/ml
S0B3603: 200 ng/ml
Free 25-OH-VD3 dilutions start from 200 ng/ml.
IC50= 3.614 ng/ml.
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | 25-OH VD3 |
| Synonyms | Calcifediol, 25-Hydroxy Vitamin D3, 25-Hydroxycholecalciferol |
| Clone Number | SDT-321-341 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | Competitive ELISA |
| Cross Reactivity | It also reacts with 25-OH-VD2 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt, 2 to 8 °C as supplied |
Background
25-hydroxyvitamin D3 (25-OH D3) is the major circulating form of vitamin D and the preferred biomarker for assessing overall vitamin D status. It is measured in serum or plasma, typically via immunoassay or liquid chromatography-tandem mass spectrometry (LC-MS/MS). This diagnostic test quantifies the body's vitamin D reserves, reflecting both cutaneous synthesis and dietary intake. It is crucial for evaluating deficiency, insufficiency, sufficiency, or toxicity. Results guide clinical decisions in managing bone health (e.g., osteoporosis, rickets), and are increasingly used in research linking vitamin D to immune, cardiovascular, and metabolic disorders. Consistent and accurate measurement remains essential for reliable diagnosis and therapeutic monitoring.
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Compete ELISA
