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VEGF 120 Protein, Mouse

VEGF 120 Protein, Mouse

Catalog Number: UA040501 Brand: UA BIOSCIENCE
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$180 USD
$180 USD
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Product Details

Product Specification


Species Mouse
Synonyms Vascular endothelial growth factor A, long form, L-VEGF, Vascular permeability factor (VPF), VEGFA
Accession Q00731
Amino Acid Sequence

Ala27-Arg146
Expression System E.coli
Molecular Weight

15-16 kDa (Reducing)
Purity >95% by SDS-PAGE; >90% by HPLC;
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Nature. 1996 Apr 4;380(6573):435-9.
Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):761-6.

Background

VEGF 120 is the smallest functional isoform of Vascular Endothelial Growth Factor A (VEGF-A) in mice, generated through alternative splicing of the Vegfa gene. Unlike the larger isoforms (VEGF 164 and VEGF 188), VEGF 120 lacks exons 6 and 7, which encode heparin-binding domains and extracellular matrix (ECM) interaction sites. This structural deficiency makes VEGF 120 the most soluble and freely diffusible isoform, unable to bind heparan sulfate proteoglycans (HSPGs) or ECM components.
The diffusible nature of VEGF 120 results in a broad, diffuse immunostaining pattern in developing tissues, contrasting with the steep, localized gradients formed by ECM-binding isoforms. In the developing mouse forebrain, VEGF 120 contributes to the dual regulation of neurogenesis and angiogenesis. Expressed by radial glia and neurons, its freely diffusible properties allow it to influence neural stem cell (NSC) proliferation and differentiation across larger tissue volumes compared to matrix-bound isoforms.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

>90% as determined by RP-HPLC.

ELISA

Immobilized VEGF-120 Protein, Mouse (Cat. No. UA040501) at 2.0μg/mL (100μL/well) can bind VEGF-R2/KDR Fc Protein, Human (Cat. No. UA010144) with EC50 of 2.92-5.46 ng/mL.