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Mycobacterium tuberculosis fbpB/Ag85B Protein, His Tag

Mycobacterium tuberculosis fbpB/Ag85B Protein, His Tag

Catalog Number: S0A2222 Brand: Starter
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Product Details

Product Specification


Synonyms Diacylglycerol acyltransferase/mycolyltransferase Ag85B, DGAT, 30 kDa extracellular protein, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex B (85B; Ag85B), Extracellular alpha-antigen, Fibronectin-binding protein B (Fbps B), MT1934
Accession P9WQP0
Amino Acid Sequence

Protein sequence (P9WQP0, Phe41-Gly325, with N-His Tag) FSRPGLPVEYLQVPSPSMGRDIKVQFQSGGNNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETFLTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGDAGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKFQDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG
Expression System HEK293
Molecular Weight Predicted MW: 32.3 kDa Observed MW: 35-43 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Ag85B is a major secreted antigen and mycolyltransferase of the Mycobacterium tuberculosis complex. This multifunctional enzyme plays a central role in constructing the unique mycobacterial cell wall. As part of the Ag85 complex, it catalyzes the transfer of mycolic acids—long-chain fatty acids—onto the arabinogalactan-peptidoglycan matrix, forming the essential mycolyl-arabinogalactan-peptidoglycan complex (mAGP). This creates the robust, hydrophobic outer membrane critical for pathogenicity and antibiotic resistance. Due to its high immunogenicity, Ag85B is also a leading candidate antigen in several tuberculosis vaccine strategies.

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SDS-PAGE

2μg（R: reducing conditions）