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IL-6 Protein, Rat

IL-6 Protein, Rat

Catalog Number: UA040507 Brand: UA BIOSCIENCE
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$100 USD
$100 USD
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Product Details

Product Specification


Species Rat
Synonyms Interleukin-6, IL-6
Accession P20607
Amino Acid Sequence

Phe25-Thr211
Expression System E.coli
Molecular Weight

20-22 kDa (Reducing)
Purity >95% by SDS-PAGE and HPLC.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized powder
Storage Buffer

20mM NaAc, 300mM NaCl, pH5.0
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Semin Immunol. 2014 Feb;26(1):2-12.
Nat Rev Rheumatol. 2014 Dec;10(12):720-7.

Background

Rat Interleukin-6 (IL-6) is a pleiotropic cytokine encoded by the Il6 gene on chromosome 4 (NC_086022.1, 5889999..5894575, complement), consisting of 5 exons that produce a protein-coding transcript . As a key member of the IL-6 family, it orchestrates acute-phase responses, immune regulation, and metabolic homeostasis in rodents. Signals through two distinct pathways in rats: Classic signaling: IL-6 binds membrane-bound IL-6Rα, recruiting gp130 to activate JAK1/STAT3 cascade; Trans-signaling: Soluble IL-6Rα (sIL-6R) complexes with IL-6 to activate gp130 on cells lacking membrane IL-6Rα, amplifying inflammatory responses in neurons and vascular tissue.

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Bioactivity

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The EC50 for this effect is 51-78pg/mL.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

RP-HPLC

>95% as determined by RP-HPLC.

ELISA

Immobilized IL-6 Protein, Rat (Cat. No. UA040507) at 5.0μg/mL (100μL/well) can bind IL-6R alpha/CD126 Fc Chimera Protein, Mouse (Cat. No. UA011252) with EC50 of 38.5-192.3 ng/mL.