Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. The EC50 is 0.286-0.831 μg/mL in the presence of 14 ng/mL human IGF-II.
IGFBP-6 Protein, Mouse
IGFBP-6 Protein, Mouse
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$120 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Insulin-like growth factor-binding protein 6, IBP-6, IGF-binding protein 6, IGFBP-6 |
| Accession | P47880 |
| Amino Acid Sequence | Ala26-Gly238 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 30-35 kDa (Reducing) |
| Purity | >95% by SDS-PAGE & HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, 1mM EDTA, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | J Clin Invest. 2008 Jul;118(7):2609-19. |
Background
Insulin-like Growth Factor Binding Protein-6 (IGFBP-6) is a 30-kDa secreted glycoprotein encoded by the Igfbp6 gene, distinguished by its extraordinary ~50–70-fold binding preference for IGF-II over IGF-I. This selectivity is mediated by cooperative interactions between the N-terminal subdomain and C-terminal domain of IGFBP-6 with adjacent binding sites on IGF-II. IGFBP-6 primarily functions as a selective inhibitor of IGF-II actions, blocking IGF-II-induced cell proliferation, differentiation, migration, and survival across diverse cell types including myoblasts, neuroblastoma cells, and rhabdomyosarcoma cells. Unlike other IGFBPs, it has minimal effect on IGF-I activity.
IGFBP-6 expression is developmentally regulated in mouse skeletal muscle, with high levels during embryogenesis and sustained expression in adult muscle tissue. In aging mice, serum IGFBP-6 levels increase progressively. Expression is regulated by TGF-β (via SMAD2/3), hedgehog signaling, p53, and hypoxia-inducible factors.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
