Standard curve
Example of Human Hyaluronidase PH-20(SPAM1)standard curve in Assay Diluent EA2.
Human Hyaluronidase PH-20(SPAM1) Onestep ELISA Kit
Human Hyaluronidase PH-20(SPAM1) Onestep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | PH-20(SPAM1) |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.4% Inter-assay: 3.7% |
| Sample type | Cell culture supernatant; Serum; Plasma; Cell culture extracts; Tissue Extracts; |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.24 ng/mL |
| Range | 1.25 ng/mL – 80 ng/mL |
| Recovery | Cell culture supernatant: 113% Serum: 108% Heparin Plasma: 102% Citrate Plasma: 117% Cell culture extracts: 106% Tissue Extracts: 107% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Hyaluronidases are a group of glycosidases catalyzing the degradation of hyaluronic acid (HA). There are six known types (hyaluronidase 1–4, PH-20, and HYALP1). Human hyaluronidase is present both in organs (testis, spleen, skin, eyes, liver, kidneys, uterus, and placenta) and body fluids (tears, blood, and semen). Testicular PH20 hyaluronidase is found on the surface of human sperm and inner acrosomal membrane and degrades hyaluronic acid in the ovum during fertilization. Hyaluronidase PH20 is known as sperm adhesion molecule 1 (SPAM1) and random hydrolysis of (1, 4)-linkages between N-acetyl- beta-D-glucosamine and D-glucuronate residues in hyaluronic acid (HA). PH20/SPAM1 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. PH20/SPAM1 protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
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ELISA
To assess the linearity of the assay, four samples were spiked with high concentrations of Human Hyaluronidase PH-20(SPAM1)
in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
