Standard curve
Example of Human H-FABP standard curve in Assay Diluent A1.
Human FABP3/H-FABP OneStep ELISA Kit
Human FABP3/H-FABP OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | H-FABP |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.1%； Inter-assay: 5.3% |
| Sample type | Cell culture supernatant; Serum; Plasma-Citrate |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.005 pg/mL |
| Range | 6.25 pg/mL – 400 pg/mL |
| Recovery | Cell culture supernatant: 89% Serum: 107% Plasma-Citrate: 103% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Fatty acid-binding protein 3 (FABP3) is a cytosolic protein found in various tissues, including heart, skeletal muscle, intestinal mucosa, liver, and kidney. It is also highly expressed in the adult brain, particularly in the pons, frontal lobe, and hippocampus. In the brain, FABP3 regulates the lipid composition of the membrane and transports fatty acids between different intracellular compartments. It is released extracellularly after neuronal damage and high cerebrospinal fluid (CSF) concentration of FABP3 is found in acute conditions, such as brain injury and stroke. CSF FABP3 concentration is also higher in conditions with neuro degeneration/neuronal damage, e.g., Alzheimer’s disease (AD), mild cognitive impairment (MCI) due to AD, dementia with Lewy bodies, and Creutzfeldt–Jakob disease. CSF FABP3 concentration correlates with cognitive decline and are associated with brain volume loss in areas selectively affected in early AD. Thus, FABP3 is suggested to be a general marker of neuronal damage.
Picture
Picture
ELISA
Linearity
The concentrations of Human H-FABP were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 4fold dilution (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.03 ng/mL in human serum.
#2 sample is undiluted samples are as follows: Human Plasma-Citrate was 4fold dilution (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.89 ng/mL in Human Plasma-Citrate.
The sample is undiluted samples are as follows: human milk was 3.2x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 12168 ng/mL in stimulated human milk sample.
Protocol Diagram
