WB result of TOP2B Recombinant Rabbit mAb
Primary antibody: TOP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 180 kDa
Observed MW: 180 kDa
TOP2B Recombinant Rabbit mAb (S-4452-44)
TOP2B Recombinant Rabbit mAb (S-4452-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TOP2B |
| Synonyms | DNA topoisomerase 2-beta |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q02880 |
| Clone Number | S-4452-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HCT 116, 293T, Jurkat, K-562, A20, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
TOP2B (DNA topoisomerase II beta) is a key nuclear enzyme ubiquitously expressed in eukaryotic cells, belonging to the type II topoisomerase family. It catalyzes the interconversion of DNA topological states by transiently cleaving and religating DNA double strands, thereby relieving supercoiling and catenation generated during DNA replication, transcription, and recombination. Unlike TOP2A, which primarily functions in DNA replication during cell proliferation, TOP2B is expressed in both non-proliferating and proliferating cells, and its activity is closely associated with gene transcription regulation—particularly during differentiation processes such as neurodevelopment, hematopoiesis, and adipogenesis, where it maintains tissue-specific gene expression programs by modulating chromatin loop structures and long-range enhancer-promoter interactions. Additionally, TOP2B participates in DNA damage repair and chromatin remodeling, and its dysfunction can lead to genomic instability. At the clinical level, TOP2B is an important target for various anticancer drugs (such as etoposide and doxorubicin), which induce DNA breaks by stabilizing the DNA-TOP2B cleavable complex, thereby killing tumor cells. However, this mechanism may also cause side effects such as therapy-related leukemia, and TOP2B dysregulation has also been associated with neurodegenerative diseases and certain developmental disorders.
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Western Blot
WB result of TOP2B Recombinant Rabbit mAb
Primary antibody: TOP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 180 kDa
Observed MW: 180 kDa
WB result of TOP2B Recombinant Rabbit mAb
Primary antibody: TOP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 180 kDa
Observed MW: 180 kDa
WB result of TOP2B Recombinant Rabbit mAb
Primary antibody: TOP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 180 kDa
Observed MW: 180 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-TOP2B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TOP2B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-TOP2B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-TOP2B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-TOP2B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
