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Syk Recombinant Rabbit mAb (S-4400-11)

Syk Recombinant Rabbit mAb (S-4400-11)

Catalog Number: S0B60264 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Syk
Synonyms Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK
Immunogen Synthetic Peptide
Location Cytoplasm, Cell membrane
Accession P43405
Clone Number S-4400-11
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu, Ms, Rt
Positive Sample Raji, SW620, A20
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt

Background

Syk (spleen tyrosine kinase) is a non-receptor tyrosine kinase widely expressed in hematopoietic cells, belonging to the Syk family. Its structural features include two tandem SH2 domains at the N-terminus, which recognize and bind to dual-phosphorylated tyrosine residues within immunoreceptor tyrosine-based activation motifs (ITAMs), thereby mediating its own activation and initiating downstream signaling cascades. It plays a central role in signal transduction through various immune receptors, including the B-cell receptor (BCR), T-cell receptor (TCR), and Fc receptors. By phosphorylating downstream substrates such as BLNK, SLP-76, and VAV, it regulates key immune responses including lymphocyte activation, proliferation, differentiation, and antibody production. Beyond adaptive immunity, Syk also participates in innate immune responses mediated by receptors such as FcγR and Dectin-1 in macrophages, neutrophils, dendritic cells, and mast cells, and plays a role in platelet adhesion and aggregation. Given its central position in immune signaling pathways, Syk dysfunction is associated with various diseases—its hyperactivation can drive allergic diseases, autoimmune disorders (such as rheumatoid arthritis and systemic lupus erythematosus), and B-cell lymphomas. Syk inhibitors (such as fostamatinib) have already been used clinically to treat immune thrombocytopenia and show promising applications in various inflammatory and oncological conditions.

Picture

Western Blot


WB result of Syk Recombinant Rabbit mAb
Primary antibody: Syk Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: SW620 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 68 kDa

WB result of Syk Recombinant Rabbit mAb
Primary antibody: Syk Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 68 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-Syk antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.