WB result of PCNA Recombinant Mouse mAb
Primary antibody: PCNA Recombinant Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDa
PCNA Recombinant Mouse mAb (S-5477)
PCNA Recombinant Mouse mAb (S-5477)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | PCNA |
| Synonyms | DNA sliding clamp PCNA; Cyclin; Proliferating cell nuclear antigen (PCNA) |
| Location | Nucleus |
| Accession | P12004 |
| Clone Number | S-5477 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, MCF7, 293T, HepG2, A431, NIH/3T3, C2C12, PC-12, C6, rat testis, rat stomach, COS-7 |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
PCNA (Proliferating Cell Nuclear Antigen) is a highly conserved DNA sliding clamp protein in eukaryotic cells. It forms a ring-shaped homotrimer composed of three identical subunits that encircles the DNA double helix like a "ring clamp," serving as a central scaffold in DNA replication and repair processes. Its core function is to act as a processivity factor for DNA polymerases—by sliding along the DNA strand, it greatly enhances the polymerase's processivity during replication, ensuring efficient and continuous DNA synthesis. At the same time, it serves as a docking platform for numerous DNA modification and repair proteins (such as key enzymes in base excision repair and mismatch repair), coordinating multiple DNA metabolic pathways. Furthermore, PCNA undergoes post-translational modifications such as ubiquitination and SUMOylation on its lysine residues, allowing it to switch functional modes under different cellular states and participate in cell cycle regulation and chromatin remodeling. Consequently, PCNA expression levels are widely used as a classic marker of cell proliferation activity in tumor pathological diagnostics. Its dysfunction or aberrant modification is closely associated with genomic instability, cancer development, and drug resistance, making it an important target in anticancer drug development.
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Western Blot
WB result of PCNA Recombinant Mouse mAb
Primary antibody: PCNA Recombinant Mouse mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDa
WB result of PCNA Recombinant Mouse mAb
Primary antibody: PCNA Recombinant Mouse mAb at 1/10000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: Rat testis lysate 20 µg
Lane 4: Rat stomach lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDa
WB result of PCNA Recombinant Mouse mAb
Primary antibody: PCNA Recombinant Mouse mAb at 1/10000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-PCNA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
