ALDOA Mouse mAb
ALDOA Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | ALDOA |
| Synonyms | Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDA; Aldolase A |
| Location | Cytoplasm |
| Accession | P04075 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, HepG2, Jurkat, NIH/3T3, 4T1, mouse liver, rat liver |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:500-1:2000 | Hu, Ms, Rt |
| ICC | 1:200-1:800 | Hu, Ms, Rt |
Background
Aldolase A, also known as fructose-bisphosphate aldolase A or ALDOA, is a crucial glycolytic enzyme primarily expressed in skeletal muscle and erythrocytes that catalyzes the reversible cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P), thereby playing a pivotal role in both glycolysis and gluconeogenesis. As a homotetrameric protein belonging to the class I aldolase family, it forms a Schiff base intermediate with its substrate via a specific lysine residue in the active site, distinguishing it mechanistically from class II aldolases that rely on metal ions. Beyond its canonical metabolic function, Aldolase A has been identified as a moonlighting protein involved in various non-glycolytic processes, including actin cytoskeleton organization, cell motility, and mRNA stability, and its dysregulation or overexpression is frequently associated with several pathological conditions, particularly various types of cancer where it supports the heightened energy demands of proliferating tumor cells through the Warburg effect, making it a potential biomarker and therapeutic target in oncology and metabolic disorders.
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Western Blot
WB result of ALDOA Mouse mAb
Primary antibody: ALDOA Mouse mAb at 1/20000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
WB result of ALDOA Mouse mAb
Primary antibody: ALDOA Mouse mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 4T1 whole cell lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 38-39 kDa
WB result of ALDOA Mouse mAb
Primary antibody: ALDOA Mouse mAb at 1/20000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 38 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
