UA-Glo® Nano luciferase split Extracellular Assay System

Sensitivity experiment of UA-Glo Nanoluc split Extracellular Assay System
HEK293 cells were seeded in 6-well culture plates, with a cell density of 80-90%. The target molecule expression plasmid with secreted type of cleavage enzyme small fragment tag was transfected. After 24 hours of transfection, the cells were resuspended in DMEM (10% FBS) medium, and added to 96-well clear cell plates with 1250 cells per well, 50 μL per well. After 24 hours, the cell plates were taken out for testing: add the UA-Glo micro cleavage luciferase extracellular detection reagent at room temperature for equilibrium for 30 minutes, and read the values and S/B (ratio of transfected cells to DMEM (10% FBS)).

Stability test of UA-Glo Nanoluc split extracellular detection reagent:
HEK293 cells were seeded in 6-well culture plates with a density of 80-90%. The target molecule expression plasmid with the secretory mitotic enzyme small fragment tag was transfected. After 24 hours of transfection, the cells were resuspended in DMEM (10% FBS) medium and added to 96-well clear cell plates with 1250 cells per well, 50 μL per well. After 24 hours, the cell plates were taken out for testing: the detection reagent was added to balance at room temperature and the detection was conducted within 10 minutes to 60 minutes, with the reading at 10 minutes set as 100% as the basis for plotting the stability graph.
The signal half-life of UA-Glo micro-mitotic luciferase extracellular detection reagent is > 2 hours.