Standard Curve
Example of His tag standard curve in Assay Diluent TE2. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
His tag Competitive OneStep ELISA Kit
His tag Competitive OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | His tag |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 8.6%； Inter-assay: 9.6% |
| Sample type | Mouse EDTA Plasma (C57BL/6); Rat EDTA Plasma (SD) |
| Assay type | Competitive (quantitative) |
| Sensitivity | 2.126 ng/mL |
| Range | 6.25 ng/mL – 400 ng/mL |
| Recovery | Mouse EDTA Plasma(C57BL/6): 98% Rat EDTA Plasma (SD): 104% |
| Assay time | 60 minutes |
| Species reactivity | Others |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. This kit is developed for rapid and high throughput detection of His-tagged proteins.
Picture
Picture
ELISA
Protocol Diagram
