Standard curve
Example of IL-4 standard curve in Assay Diluent .
Human IL-4 Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-4 Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-4 |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3%； Inter-assay: 5% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.1 pg/mL |
| Range | 0.43 pg/mL-3500 pg/mL |
| Recovery | Plasma: 110% Serum: 106% Cell culture supernatant: 122% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Interleukin 4 (IL-4) was first identified as a helper T cell product with the capacity to co-stimulate B cell growth in vitro. IL-4 also can rescue B-cells from apoptosis, enhancing their survival, and is responsible for immunoglobulin isotype switching to IgG1 and IgE. The effect of IL-4 signaling is mediated through the IL-4 receptor alpha chain (IL-4Rα). Upon binding to its ligand, IL-4Rα dimerizes either with the common gamma chain (γc) to produce the type-1 signaling complex located mainly on hematopoietic cells, or with the IL-13 receptor alpha 1 (IL-13Rα1) to produce the type-2 complex, which is expressed also on non-hematopoietic cells. The type-1 signaling complex is critical for Th2-skewing of T cells and the development of alternatively activated macrophages (AAMΦs), while the type-2 complex plays a role in non-hematopoietic responses to IL-4 and IL-13.
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CBA
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of IL-4 in the supernatant was measured to be 36.22 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-4 in the supernatant was measured to be 1.47 pg/mL.
Linearity
The high-concentration human IL-4 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human IL-4 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
