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TRAF2 Rabbit Polyclonal Antibody

TRAF2 Rabbit Polyclonal Antibody

Catalog Number: S0B6043 Application: WB,ICC Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Regular price $70.00 USD
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Product Details

Product Specification


Host Rabbit
Antigen TRAF2
Synonyms TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; RING-type E3 ubiquitin transferase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3; TRAP3
Immunogen Synthetic Peptide
Location Cytoplasm
Accession Q12933
Antibody Type Polyclonal antibody
Isotype IgG
Application WB, ICC
Reactivity Hu
Positive Sample HeLa, HEK-293, 293T, Molt-4
Purification Immunogen Affinity
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
ICC 1:500 Hu

Background

TNF receptor-associated factor 2 (TRAF2) is a multifunctional protein that plays a crucial role in mediating signal transduction from members of the TNF receptor superfamily. It is a 56 kDa trimeric protein, consisting of a globular C-terminal TRAF domain and an extended coiled-coil N-terminal region. TRAF2 acts as both an adaptor protein and an E3 ubiquitin ligase, facilitating the activation of downstream signaling pathways such as the JNK/c-Jun and IKK/NF-κB pathways in response to TNFα stimulation. It directly interacts with TNF receptors and forms complexes with other TRAF proteins, such as TRAF1, to mediate anti-apoptotic signals and inhibit caspase activation. Additionally, TRAF2 is involved in various physiological processes, including cell survival, proliferation, and stress responses, and it has been implicated in cancer progression and immune regulation.

Picture

Western Blot

WB result of TRAF2 Rabbit pAb
Primary antibody: TRAF2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 52 kDa

Immunocytochemistry

ICC shows positive staining in HEK293 cells. Anti-TRAF2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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