| Host | Rabbit |
| Antigen | TCL1 |
| Synonyms | Oncogene TCL-1, Protein p14 TCL1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P56279 |
| Clone Number | SDT-052-12 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IHC-P | 1:2000 | |
| WB | 1:1000 | |
| IP | 1:25 |
T-cell leukemia/lymphoma protein 1A is a protein that in humans is encoded by the TCL1A gene. The TCL1 locus on human chromosome 14q32.1 is activated in T-cell leukemias by translocations and inversions that juxtapose it to regulatory elements of T-cell receptor genes. TCL1A has been shown to interact with AKT1 and AKT2. Protein expression in immature T and B lymphoid cells and overexpression of TCL1 is considered critical in the oncogenetic transformation.
WB result of TCL1 Rabbit mAb
Primary antibody: TCL1 Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: Daudi whole cell lysate 20 µg
Negative control: HL-60 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 13 kDa
Exposure time: 6s
TCL1 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating TCL1 in 0.4mg Daudi whole cell lysate. Western blot was performed on the immunoprecipitate using TCL1 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: Daudi whole cell lysate 10µg (input)
Lane 2: TCL1 Rabbit mAb IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
Predicted MW: 13 kDa
Observed MW: 13 kDa
Exposure time: 72s
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-TCL1 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen.
Anti-TCL1 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
