IHC shows positive staining in paraffin-embedded human kidney. Anti-Renin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Renin Recombinant Rabbit mAb (SDT-087-48)
Renin Recombinant Rabbit mAb (SDT-087-48)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Renin |
| Synonyms | Angiotensinogenase |
| Immunogen | Recombinant Protein |
| Location | Secreted, Membrane |
| Accession | P00797 |
| Clone Number | SDT-087-48 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | null |
Background
Renin is a central hormone in the control of blood pressure and various other physiological functions. The main source of renin is the juxtaglomerular cells (JGCs), which release renin from storage granules [PMID: 12949225].
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Renin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Renin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
