WB result of Phospho-MLKL (Ser345) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-MLKL (Ser345) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mouse TNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 54 kDa
Phospho-MLKL (Ser345) Recombinant Rabbit mAb (S-3552)
Phospho-MLKL (Ser345) Recombinant Rabbit mAb (S-3552)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-MLKL (Ser345) |
| Synonyms | Mixed lineage kinase domain-like protein; Mlkl |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | Q9D2Y4、Q8NB16 |
| Clone Number | S-3552 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Phospho-MLKL (Ser345) is the activated, phosphorylated form of the pseudokinase mixed-lineage kinase domain-like protein (MLKL) at serine-345, a critical post-translational modification catalyzed by the necroptotic kinase RIPK3 that triggers MLKL’s oligomerization, translocation to plasma membrane phosphoinositides, and subsequent membrane disruption, thereby executing the terminal, pro-inflammatory cell death pathway known as necroptosis; detection of phospho-MLKL(Ser345) by phospho-specific antibodies thus serves as a specific biomarker for ongoing necroptotic signaling in experimental models and human pathologies including ischemia–reperfusion injury, inflammatory diseases, and certain cancers.
Picture
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Western Blot
