WB result of IGSF8 Recombinant Rabbit mAb
Primary antibody: IGSF8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 72 kDa
This blot was developed with high sensitivity substrate
IGSF8 Recombinant Rabbit mAb (S-2101-8)
IGSF8 Recombinant Rabbit mAb (S-2101-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IGSF8 |
| Synonyms | Immunoglobulin superfamily member 8; CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2 (EWI-2); Keratinocytes-associated transmembrane protein 4 (KCT-4); LIR-D1; Prostaglandin regulatory-like protein (PGRL); CD316; CD81P3; EWI2; KCT4 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q969P0 |
| Clone Number | S-2101-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, Jurkat, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Immunoglobulin superfamily member 8 (IGSF8), also known as CD316, is a protein encoded by the IGSF8 gene in humans. It is an innate immune checkpoint that inhibits the cytotoxic activity of natural killer (NK) cells by binding to the Killer Ig-like Receptor KIR3DL2. IGSF8 is a member of the EWI subfamily of the immunoglobulin protein superfamily, containing a single transmembrane domain, an EWI motif, and a variable number of immunoglobulin domains. It interacts with tetraspanins CD81 and CD9, regulating their roles in various cellular functions, including cell migration and viral infection. IGSF8 may also function as a tumor suppressor by inhibiting the proliferation of certain cancers.
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Western Blot
WB result of IGSF8 Recombinant Rabbit mAb
Primary antibody: IGSF8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
WB result of IGSF8 Recombinant Rabbit mAb
Primary antibody: IGSF8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
