Standard curve
Example of IL-6 standard curve in Assay Diluent .
Human IL-6 Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-6 Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-6 |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3%； Inter-assay: 5% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.05 pg/mL |
| Range | 0.61 pg/mL-5000 pg/mL |
| Recovery | Plasma: 98% Serum: 97% Cell culture supernatant: 112% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
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CBA
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of IL-6 in the supernatant was measured to be 4256.01 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-6 in the supernatant was measured to be 170.03 pg/mL.
Linearity
The high-concentration human IL-6 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human IL-6 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
