Standard curve
Example of IL-5 standard curve in Assay Diluent .
Human IL-5 Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-5 Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-5 |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3%； Inter-assay: 5% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.34 pg/mL |
| Range | 1.07 pg/mL-8750 pg/mL |
| Recovery | Plasma: 108% Serum: 94% Cell culture supernatant: 113% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
IL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.
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CBA
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of IL-5 in the supernatant was measured to be 310.23 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-5 in the supernatant was measured to be 0.15 pg/mL.
Linearity
The high-concentration human IL-5 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human IL-5 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
