Flow cytometric analysis of Mouse CD335 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse NK1.1 antibody and either FITC Rat IgG2a, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse CD335 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse CD335 Antibody (S-R613)
FITC Rat Anti-Mouse CD335 Antibody (S-R613)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD335 |
| Synonyms | Natural cytotoxicity triggering receptor 1; Activating receptor 1 (mAR-1); Lymphocyte antigen 94; Natural killer cell p46-related protein (NK-p46; NKp46; mNKp46); Ncr1; Ly94 |
| Location | Cell membrane |
| Accession | Q8C567 |
| Clone Number | S-R613 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
CD335, also known as NKp46, is a 46kDa natural cytotoxicity receptor that belongs to the immunoglobulin superfamily. It is expressed on resting and activated NK cells, ILC1, a subset of NCR+ ILC3 in the mucosa, and a small subset of T lymphocytes. CD335 plays a crucial role in the lysis of autologous, allogeneic, or xenogeneic cells by NK cells and is highly conserved in mammals.
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