Flow cytometric analysis of SD Rat splenocytes treated overnight with 3 μg/ml ConA (Right panel) or untreated (Left panel) was stained with APC-Cy7 Mouse Anti-Rat CD4 Antibody and SDT FITC Mouse Anti-Rat CD25 Antibody at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Rat CD25 Antibody (S-R676)
FITC Mouse Anti-Rat CD25 Antibody (S-R676)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD25 |
| Synonyms | Peripheral myelin protein 22; PMP-22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Pmp22 |
| Location | Cell membrane |
| Accession | P25094 |
| Clone Number | S-R676 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
CD25, also known as IL2RA, is the alpha chain of the high-affinity interleukin-2 receptor, which is a heterotrimeric complex that also includes beta (IL2RB) and the common gamma chain (IL2RG). This receptor interacts with interleukin-2, a cytokine crucial for immune homeostasis. CD25 is typically expressed on activated T lymphocytes and plays a significant role in their proliferation and function. It is also found on certain B cells, where its expression can be upregulated by specific Toll-like receptor ligands. In addition to its role in the immune system, CD25 has potential as a therapeutic target in cancer treatment due to its expression on various tumor cells.
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