| Host | Rabbit |
| Antigen | CD31 |
| Synonyms | PECAM-1,EndoCAM, GPIIA, PECA1 |
| Immunogen | Synthetic Peptide |
| Accession | P16284 |
| Clone Number | SDT-008-17 |
| Antibody Type | Rabbit mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Research Area | Immunology |
| Concentration | 0.5mg/ml |
| Molecular Weight | 150kDa |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IHC-P | 1:250 | |
| IP | 1:25 | |
| WB | 1:1000 |
Cluster of differentiation 31 (CD31) also known as platelet endothelial cell adhesion molecule (PECAM-1) is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. This can help to evaluate the degree of tumor angiogenesis, which can imply a rapidly growing tumor. Malignant endothelial cells also commonly retain the antigen, so that CD31 immunohistochemistry can also be used to demonstrate both angiomas and angiosarcomas. It can also be demonstrated in small lymphocytic and lymphoblastic lymphomas, although more specific markers are available for these conditions.
WB result of anti-CD31 antibody
Primary antibody : Anti-CD31 antibody at 1/1000 dilution
Lane 1 : Hela whole cell lysate 20 µg
Lane 2 : THP1 whole cell lysate 20 µg
Negative control: Hela whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 150 kDa
econds
CD31 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD31 in 0.4mg THP-1 whole cell lysate. Western blot was performed on the immunoprecipitate using CD31 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: THP-1 whole cell lysate 10µg (input)
Lane 2: CD31 Rabbit mAb IP in THP-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in THP-1 whole cell lysate
Predicted MW: 82 kDa
Observed MW: 150 kDa
IHC shows membrane staining in paraffin-embedded human kidney endothelial cells.
Anti-CD31 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human placenta endothelial cells.
Anti-CD31 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human lung squamous cell cancer endothelial cells.
Anti-CD31 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human hepatocellular carcinoma endothelial cells.
Anti-CD31 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
